Gel electrophoresis and subsequent staining is a routine procedure prior to protein identification. Most often SDS-Gels are used. If you don’t use commercial gels, it is of utmost importance that gel-hardening is completed over night.
Not all coomassie stains are compatible with mass spectrometry. We strongly recommend that you mix your own coomassie staining solution directly prior to use. Alternatively, visualization can be performed with silver staining when protein concentration is very low.
Cutting of gel bands
Please take precautions to prevent contamination (keratins, etc) when cutting gel bands and consider the following recommendations:
Alternatively, the whole gel can be transferred to the PCF.