USERS GUIDE

 

DO

  • Fill out the request form and discuss your question before sending samples
  • Contact the Facility at any time to discuss sample preparation / processing / shipping
  • Provide us with as much information about your sample / protein as you can
  • Avoid contaminations

· Use clean containers to stain and store gels, and use them only for this purpose

· Use a lid for the container mentioned above

· Wear gloves at all times during sample handling

· Cut gel bands on a clean bench and a clean glass plate using a clean (=new) scalpel knife.

  • Acknowledge the Facility in your publications (please), if analyses / services have contributed to your work

 

DON'T

  • DON'T ship samples over the weekend
  • DON'T incubate gels in the microwave to expedite coomassie staining
  • DON'T use commercial kits for coomassie staining
  • DON'T use parafilm at all
  • DON'T use overhead transparencies to scan gels
  • DON'T use detergents for MS experiments of intact proteins
  • DON'T use >5% glycerol for MS experiments of intact proteins